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vesicular stomatitis virus g vsv g envelope expression vector  (Addgene inc)


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    Structured Review

    Addgene inc vesicular stomatitis virus g vsv g envelope expression vector
    Vesicular Stomatitis Virus G Vsv G Envelope Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14951 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vesicular+stomatitis+virus+g+vsv+g+envelope+expression+vector/pMD2%2EG+(Plasmid+%2312259)/pm39389033-261-81-90
    Average 98 stars, based on 14951 article reviews
    vesicular stomatitis virus g vsv g envelope expression vector - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Knock-Out:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Polymerase Chain Reaction:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Recombinant:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Clone Assay:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Sequencing:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Plasmid Preparation:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Virus:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Expressing:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Derivative Assay:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.

    Transfection:

    Article Title: MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes
    Article Snippet: All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) GlutaMAX supplement (#10566016, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS, #10270106, Thermo Fisher Scientific), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Article Title: MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
    Article Snippet: WPRE (Addgene; Cat# 12252)111 N/A HIV-1 clone pNL4-3.Luc.R-E- Obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH contributed by Dr. Nathaniel Landau.



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    Addgene inc vesicular stomatitis virus glycoprotein vsv g envelope expression vector
    Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with <t>VSV-G</t> pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.
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    Image Search Results


    Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with VSV-G pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.

    Journal: Viruses

    Article Title: Efficient Inhibition of HIV Using CRISPR/Cas13d Nuclease System

    doi: 10.3390/v13091850

    Figure Lengend Snippet: Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with VSV-G pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.

    Article Snippet: The CasRx, (pXR001; Addgene #109049), dCasRx (PXR002; Addgene #109050) plasmids, gRNA expression vectors (pXR003, pXR004; Addgene #109053 and #109054), Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2; Addgene # 12260), iRFP670 fluorescent reporter vector (piRFP670-N1; Addgene#79987) and pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene #67974) were obtained from the Addgene repository (Watertown, MA, USA).

    Techniques: Inhibition, Transfection, Plasmid Preparation, Infection, Expressing, Flow Cytometry, Two Tailed Test